By M.H. Assareh, K. Jaimand and M.B. Rezaee

Abstract: Abstract: The essential oils isolated by hydrodistillation from the leaves of six Eucalyptus were analyzed by GC and GC/MS. The main components identified in E. intertexta R.T.Baker oil were 1,8-cineole (64.8%), terpinen-1-ol (7.2%) and α-pinene (5.7%); in E. largiflorens F.Muell. were 1,8-cineole (47%), p-cymene (10.6%) and α-terpineol (8.5%); in E. kingsmillii Maiden et Blakely were 1,8-cineole (77%), α-pinene (8.7%) and camphene (3.8%); in E. dealbata A. Cunn. were 1,8-cineole (70.6%), α-pinene (13%) and terpinen-1-ol (3.7%). The major components of the oil of E. loxophleba Benth. ssp. loxophleba were 1,8-cineole (41.9%), α-pinene (13.7%) and aromadendrene (3.7%), and the major components of E. kruseana F.Muell. were bicyclogermacrene (28.8%), α-pinene (17.7%) and 1,8-cineole (12.1%). Key Word Index: Eucalyptus intertexta, Eucalyptus largiflorens, Eucalyptus kingsmillii, Eucalyptus dealbata, Eucalyptus loxophleba subsp. loxophleba, Eucalyptus kruseana, Myrtaceae, essential oil compostion, 1,8-cineole, α-pinene.

Plant Name
Eucalyptus intertexta R.T.Baker, Eucalyptus largiflorens F.Muell., Eucalyptus kingsmillii Maiden et Blakely, Eucalyptus dealbata A. Cunn., Eucalyptus loxophleba Benth. ssp. loxophleba and Eucalyptus kruseana F.Muell.

Source
The seeds of the six Eucalyptus species: E. intertexta, E. largiflorens, E. kingsmillii, E. dealbata, E. loxophleba ssp. loxophleba and E. kruseana were purchased from the trial plots established at the propagated from seeds supplied by the Kimberly, Seed Co. Perth, Australia in 1993. The plants were cultivated in 1994 southwest of Iran in Dezful city in Fadak research station with ecological condition of latitude 16° 32´, longitude 25° 48´, elevation 80 m, precipitation 250 mm, max temp. 52°C and min. temp. -2°C, soil texture and sandy loam.

Plant Part
About 35 g of leaves from trees of the three Eucalyptus species collected on April 2004 were air-dried and subjected to hydrodistillation for 20 min using a Clevenger-type apparatus. The oils were separated from the water by decantation and were dried by filtration over anhydrous sodium sulfate. Oil yield for E. intertexta 0.48%, E. largiflorens 0.82%, E. kingsmillii 2.05%, E. dealbata 1.22%, E. loxophleba ssp. loxophleba 2.11% and E. kruseana 1.02%.

Introduction
The genus Eucalyptus (family Myrtaceae) comprises well known plants of over 600 species of trees (1). Although most of the plants are native to Australia, numerous species have been introduced to other parts of the world, including Iran, as economic and ornamental trees in forest trial provenances (2) where the plants have become source of important fast-growing hardwood trees (3) and Eucalyptus oils (4). The Eucalyptus essential oils could be grouped into three types (medicinal, industrial and perfumery) on the basis of their chemical constituents. Consequently, Eucalyptus oil compositions from various countries have been extensively investigated due to their numerous uses in the pharmaceutical and cosmetics industries. Previous studies of the leaf oil compositions of Eucalyptus species used commercially as a natural source of 1,8-cineole have been reported (5–7). The major component of the leaf oil of E. kingsmillii (9), E. dealbata (9), E. loxophleba (10) E. largiflorens (6), E. intertexta (6,7) and E. kruseana (11,12) was reported to be 1,8-cineole. Much research has been done on the oil composition of different Eucalyptus species. The oils of some of these Eucalyptus species and their chemical constituents were not investigated in Iran. Practically nothing whatsoever is known about the constituents of the Iran-grown Eucalyptus species, in spite of their important industrial uses. It was therefore necessary to investigate the composition of the oils of these plants and correlate the economic potential of the oils. This paper now reports, for the first time, the results of the analyses of leaf oils of E. intertexta, E. largiflorens, E. kingsmillii, E. dealbata, E. loxophleba ssp. loxophleba and E. kruseana growing in Iran.